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GWAS Study

Whole-genome sequencing in diverse subjects identifies genetic correlates of leukocyte traits: The NHLBI TOPMed program.

Mikhaylova AV, McHugh CP, Polfus LM et al.

34582791 PubMed ID
GWAS Study Type
260928 Participants
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Chapter I

Publication Details

Comprehensive information about this research publication

Authors

MA
Mikhaylova AV
MC
McHugh CP
PL
Polfus LM
RL
Raffield LM
BM
Boorgula MP
BT
Blackwell TW
BJ
Brody JA
BJ
Broome J
CN
Chami N
CM
Chen MH
CM
Conomos MP
CC
Cox C
CJ
Curran JE
DM
Daya M
EL
Ekunwe L
GD
Glahn DC
HN
Heard-Costa N
HH
Highland HM
HB
Hobbs BD
IY
Ilboudo Y
JD
Jain D
LL
Lange LA
MT
Miller-Fleming TW
MN
Min N
MJ
Moon JY
PM
Preuss MH
RJ
Rosen J
RK
Ryan K
SA
Smith AV
SQ
Sun Q
SP
Surendran P
DV
de Vries PS
WK
Walter K
WZ
Wang Z
WM
Wheeler M
YL
Yanek LR
ZX
Zhong X
AG
Abecasis GR
AL
Almasy L
BK
Barnes KC
BT
Beaty TH
BL
Becker LC
BJ
Blangero J
BE
Boerwinkle E
BA
Butterworth AS
CS
Chavan S
CM
Cho MH
CH
Choquet H
CA
Correa A
CN
Cox N
DD
DeMeo DL
FN
Faraday N
FM
Fornage M
GR
Gerszten RE
HL
Hou L
JA
Johnson AD
JE
Jorgenson E
KR
Kaplan R
KC
Kooperberg C
KK
Kundu K
LC
Laurie CA
LG
Lettre G
LJ
Lewis JP
LB
Li B
LY
Li Y
LD
Lloyd-Jones DM
LR
Loos RJF
MA
Manichaikul A
MD
Meyers DA
MB
Mitchell BD
MA
Morrison AC
ND
Ngo D
ND
Nickerson DA
NS
Nongmaithem S
NK
North KE
OJ
O'Connell JR
OV
Ortega VE
PN
Pankratz N
PJ
Perry JA
PB
Psaty BM
RS
Rich SS
SN
Soranzo N
RJ
Rotter JI
SE
Silverman EK
SN
Smith NL
TH
Tang H
TR
Tracy RP
TT
Thornton TA
VR
Vasan RS
ZJ
Zein J
MR
Mathias RA
RA
Reiner AP
AP
Auer PL
Chapter II

Abstract

Summary of the research findings

Many common and rare variants associated with hematologic traits have been discovered through imputation on large-scale reference panels. However, the majority of genome-wide association studies (GWASs) have been conducted in Europeans, and determining causal variants has proved challenging. We performed a GWAS of total leukocyte, neutrophil, lymphocyte, monocyte, eosinophil, and basophil counts generated from 109,563,748 variants in the autosomes and the X chromosome in the Trans-Omics for Precision Medicine (TOPMed) program, which included data from 61,802 individuals of diverse ancestry. We discovered and replicated 7 leukocyte trait associations, including (1) the association between a chromosome X, pseudo-autosomal region (PAR), noncoding variant located between cytokine receptor genes (CSF2RA and CLRF2) and lower eosinophil count; and (2) associations between single variants found predominantly among African Americans at the S1PR3 (9q22.1) and HBB (11p15.4) loci and monocyte and lymphocyte counts, respectively. We further provide evidence indicating that the newly discovered eosinophil-lowering chromosome X PAR variant might be associated with reduced susceptibility to common allergic diseases such as atopic dermatitis and asthma. Additionally, we found a burden of very rare FLT3 (13q12.2) variants associated with monocyte counts. Together, these results emphasize the utility of whole-genome sequencing in diverse samples in identifying associations missed by European-ancestry-driven GWASs.

14,246 African American individuals, 686 East Asian ancestry individuals, 33,285 European American ancestry individuals, 13,585 Hispanic/Latin American individuals

Chapter III

Study Statistics

Key metrics and study information

260928
Total Participants
GWAS
Study Type
Yes
Replicated
199,126 African ancestry, European ancestry, Hispanic or Latin American individuals
Replication Participants
African unspecified, East Asian, European, Hispanic or Latin American, African unspecified, European, Hispanic or Latin American
Ancestry
U.S., U.K.
Recruitment Country
Chapter IV

Analysis

Comprehensive review of health and genetic findings

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Analysis In Progress

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