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GWAS Study

Genetic Link Determining the Maternal-Fetal Circulation of Vitamin D.

Sampathkumar A, Tan KM, Chen L et al.

34621292 PubMed ID
GWAS Study Type
812 Participants
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Chapter I

Publication Details

Comprehensive information about this research publication

Authors

SA
Sampathkumar A
TK
Tan KM
CL
Chen L
CM
Chong MFF
YF
Yap F
GK
Godfrey KM
CY
Chong YS
GP
Gluckman PD
RA
Ramasamy A
KN
Karnani N
Chapter II

Abstract

Summary of the research findings

Vitamin D is an essential micronutrient whose demand is heightened during pregnancy to support the growth of the fetus. Furthermore, the fetus does not produce vitamin D and hence relies exclusively on the supply of maternal vitamin D through the placenta. Vitamin D inadequacy is linked with pregnancy complications and adverse infant outcomes. Hence, early predictive markers of vitamin D inadequacy such as genetic vulnerability are important to both mother and offspring. In this multi-ethnic Asian birth cohort study, we report the first genome-wide association analysis (GWAS) of maternal and fetal vitamin D in circulation. For this, 25-hydroxyvitamin D (25OHD) was measured in the antenatal blood of mothers during mid gestation (n=942), and the cord blood of their offspring at birth (n=812). Around ~7 million single nucleotide polymorphisms (SNPs) were regressed against 25OHD concentrations to identify genetic risk variants. About 41% of mothers had inadequate 25OHD (≤75nmol/L) during pregnancy. Antenatal 25OHD was associated with ethnicity [Malay (Β=-22.32nmol/L, p=2.3×10-26); Indian (Β=-21.85, p=3.1×10-21); reference Chinese], age (Β=0.47/year, p=0.0058), and supplement intake (Β=16.47, p=2.4×10-13). Cord blood 25OHD highly correlated with antenatal vitamin D (r=0.75) and was associated with ethnicity [Malay (Β=-4.44, p=2.2×10-7); Indian (Β=-1.99, p=0.038); reference Chinese]. GWAS analysis identified rs4588, a missense variant in the group-specific component (GC) gene encoding vitamin D binding protein (VDBP), and its defining haplotype, as a risk factor for low antenatal (Β=-8.56/T-allele, p=1.0×10-9) and cord blood vitamin D (Β=-3.22/T-allele, p=1.0×10-8) in all three ethnicities. We also discovered a novel association in a SNP downstream of CYP2J2 (rs10789082), a gene involved in 25-hydroxylation of vitamin D, with vitamin D in pregnant women (Β=-7.68/G-allele, p=1.5×10-8), but not their offspring. As the prevention and early detection of suboptimal vitamin D levels are of profound importance to both mother and offspring's health, the genetic risk variants identified in this study allow risk assessment and precision in early intervention of vitamin D deficiency.

up to 399 Chinese ancestry neonates, up to 166 Indian ancestry neonates, up to 247 Malay ancestry neonates

Chapter III

Study Statistics

Key metrics and study information

812
Total Participants
GWAS
Study Type
No
Replicated
East Asian, South Asian, South East Asian
Ancestry
Singapore
Recruitment Country
Chapter IV

Analysis

Comprehensive review of health and genetic findings

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